Record Display for the EPA National Library Catalog

RECORD NUMBER: 10 OF 17

OLS Field Name OLS Field Data
Main Title Human Epithelial Cell Activation Systems.
Author McCormick, J. Justin ;
CORP Author Michigan State Univ., East Lansing. Carcinogenesis Lab.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1982
Report Number EPA-R-8055630; EPA-600/1-82-015;
Stock Number PB83-114264
Subjects Humans ; Chromatographic analysis ; Culture media ; Freezing ; Hydrocarbons ; Metabolism ; Bioassay ; Deoxyribonucleic acid ; Carcinogens ; Carcinogenesis ; Cell cultures ; Mutagenesis ; Cell lines ; HPLC ; Biological effects
Additional Subjects Humans ; Chromatographic analysis ; Culture media ; Freezing ; Hydrocarbons ; Metabolism ; Bioassay ; Deoxyribonucleic acid ; Carcinogens ; Carcinogenesis ; Cell cultures ; Mutagenesis ; Cell lines ; HPLC ; Biological effects
Holdings
Library Call Number Additional Info Location Last
Modified
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Status
NTIS  PB83-114264 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/23/1988
Collation 38p
Abstract
Much effort has been placed on the development of short-term assay systems that use the induction of mutations in bacterial and mammalian cells as the basis for predicting the carcinogenicity of environmental chemicals. However, evidence has accumulated that suggests that most environmental chemicals require enzymatic activation to produce metabolites that will react with cellular macromolecules, and many or the bacterial and mammalian cell lines identified are unable to produce that activation. Consequently, scientists began to use microsomal systems to supply activation. In this study, two short-term assays were examined and cells that appeared capable of metabolizing various carcinogens were identified. In the first method, the metabolism of tritiated benzo(a)pyrene (B(a)P) to aqueous-acetone soluble products was measured. While this method is useful to identify cells that are capable of metabolizing B(a)P or related compounds, it is not applicable to other classes of compounds. Therefore, a second, more general method was examined. This second method detects the formation of agents that damage DNA by measuring DNA synthesis inhibition after exposure to a carcinogen.