| Main Title |
Mutagenicity testing in mammalian cells : multiple drug-resistance markers / |
| Author |
Carver, June H. ;
Adair, Gerald M. ;
Wandres, Daniel L. ;
Carver, J. H. ;
Adair, G. M. ;
Wandres, D. L.
|
| Other Authors |
|
| CORP Author |
California Univ., Livermore. Lawrence Livermore Lab.;Environmental Protection Agency, Washington, DC. |
| Publisher |
Office of Energy, Minerals and Industry, U.S. Environmental Protection Agency, |
| Year Published |
1979 |
| Report Number |
EPA-600/7-79-173; UCID-18599; EPA-W-7405-eng-48 |
| Stock Number |
PB80-183114 |
| OCLC Number |
53964424 |
| Subjects |
Cell culture ;
Mammals ;
Mutagenicity testing
|
| Additional Subjects |
Mutations ;
Cells(Biology) ;
Mammals ;
In vitro analysis ;
Screening ;
Tests ;
Nitrogen organic compounds ;
Sulfur organic compounds ;
Mutagens ;
Mutagenesis ;
Cell lines ;
Biological effects ;
Imidazotriazine/amino ;
Thioguanine ;
Uridine/fluoro-deoxy ;
Benzopyrenes ;
Quabain ;
Sulfonic acid/methyl ;
Guanidine/N-methyl-N-nitro-nitroso ;
Quinaline oxide/nitro ;
Nitrosamine/dimethyl ;
Mutagen screening ;
Pollution ;
Animal cells ;
Cell cultures ;
Evaluation ;
Hamsters ;
Performance testing ;
ERDA/560301
|
| Internet Access |
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| EJED |
EPA 600/7-79-173 |
|
OCSPP Chemical Library/Washington,DC |
01/23/2004 |
| EKBD |
EPA-600/7-79-173 |
|
Research Triangle Park Library/RTP, NC |
01/02/2004 |
| ELBD ARCHIVE |
EPA 600-7-79-173 |
Received from HQ |
AWBERC Library/Cincinnati,OH |
10/04/2023 |
| ERAD |
EPA 600/7-79-173 |
|
Region 9 Library/San Francisco,CA |
02/11/2013 |
| ESAD |
EPA 600-7-79-173 |
|
Region 10 Library/Seattle,WA |
03/23/2010 |
| NTIS |
PB80-183114 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
| NTIS |
UCID-18599 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
xiii, 61 pages : charts ; 28 cm. |
| Abstract |
Chinese hamster ovary (CHO) cell lines heterozygous at the adenine phosphoribosyltransferase (aprt) and thymidine kinase (tk) loci were isolated and used for single-step selection of spontaneous and induced mutants resistant to 8-azaadenine (AAr), 6-thioguanine (TGr), 5-fluorodeoxyuridine (FUdRr), or ouabain (OUAR). Mutation data are reported for direct mutagens ethyl methanesulfonate (EMS), N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), 4-nitroquinoline-1-oxide (NQO) and for promutagens dimethylnitrosamine (DMN) and benzo(a)pyrene (BaP) activated by rat liver homogenates. Critical plating densities were established for AAr, TGr, and FUdRr. Optimal expression of mutant phenotypes after mutation induction with EMS, DMN, or BaP were 2 to 4 d for AAr, 6 to 8 d for TGr, 3 d for OUAR, and 1 to 3 d for FUdRr. The induced mutant frequencies as a function of relative cell survival after treatment with EMS, DMN, or BaP showed locus-specific differences in sensitivity. Of 61 clonal isolates resistant to AA and assayed for APRT activity, 87% had < or = 5% wild type activity; of 30 TGr clones assayed, 83% had < or = 5% wild type HGPRT activity. Of 42 FUdRr clones assayed, 41 had <1% wild type TK activity. Fifty clones selected in medium containing FUdR displayed cross-resistance to 5-bromodeoxyuridine (BUdR) and trifluorothymidine (TFT) and all were sensitive to HAT (amethopterin-hypoxanthine-thymidine) medium. The tk locus showed the largest mutational response as a function of relative cell survival after mutagen treatment. |
| Notes |
EPA Project Officer: G. Rausa. U.S.D.O.E. Project Director: G. Stapleton. Biomedical Sciences Division, Lawrence Livermore Laboratory, University of California. Contract Number: Dept. of Energy Contract Number W-7405-ENG-48 "August 1979." Includes bibliographical references (pages 52-60). "UCID-18599." "EPA-600/7-79-173." |
