Record Display for the EPA National Library Catalog


Main Title Introduction of a Ha-ras Oncogene into Rat Liver Epithelial Cells and Parenchymal Hepatocytes Confers Resistance to the Growth Inhibitory Effects of TGF-Beta.
Author Houck, K. A. ; Michalopoulos, G. K. ; Strom, S. C. ;
CORP Author Duke Univ. Medical Center, Durham, NC. Dept. of Pathology. ;Virginia Commonwealth Univ., Richmond.;Health Effects Research Lab., Research Triangle Park, NC.
Publisher c1989
Year Published 1989
Report Number EPA-R-814344; EPA/600/J-89/203;
Stock Number PB90-147794
Additional Subjects Liver ; Epithelium ; Cells(Biology) ; Deoxyribonucleic acids ; Reprints ; Transforming growth factors ; Transfection ; Oncogenes ; Protooncogenes ; Autoradiography ; Ras genes ; Southern immunoblotting
Library Call Number Additional Info Location Last
NTIS  PB90-147794 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 9p
Growth of rat liver epithelial cells (RLEC) and primary cultures of parenchymal hepatocytes is potently inhibited by TGF-Beta. Transfection of a mutated Ha-ras oncogene, but not a human c-myc oncogene, into RLEC resulted in cell lines resistant to growth inhibition by TGF-Beta under anchorage-dependent conditions. Infection of primary rat hepatocyte cultures with v-Ha-ras yielded a cell line likewise insensitive to inhibition by TGF-Beta. Binding of (125I)TGF-Beta to Ha-ras-transfected RLEC was reduced relative to control or c-myc-transfected cells. These data suggest that activation of a Ha-ras oncogene in epithelial cells may result in escape from negative growth control and hence be a critical step during carcinogenesis. (Copyright (c) 1989 The MacMillan Press Ltd.)