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Main Title Composition of Glycoproteins Secreted by Tracheal Explants from Various Animal Species.
Author Kaizu, Tokio ; Lyons, Sheila A. ; Cross, Carroll E. ; Jennings, Mark D. ; Last, Jerold A. ;
CORP Author California Primate Research Center, Davis.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1979
Report Number EPA-R-805355; EPA-600/J-82-207;
Stock Number PB82-250614
Additional Subjects Glycoproteins ; Trachea ; Laboratory animals ; Chromatography ; Electrophoresis ; Humans ; Bronchi ; Mucus ; Reprints ;
Holdings
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Status
NTIS  PB82-250614 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 8p
Abstract
The acidic and neutral glycoproteins secreted by cultured tracheal explants from pigs, sheep, rats, mice, monkeys, guinea pigs, dogs, and chickens were purified and fractionated by column chromatography on DEAE-cellulose and by electrophoresis on cellulose acetate. The ratios of acidic to neutral mucus glycoproteins were compared for the above animals with that of mucus glycoproteins secreted by cultured human bronchi. The observed ratios of acidic to neutral glycoproteins ranged from 4.0 (mouse) to 7.2 (chicken and pig) from cultured tracheae; secreted human bronchial mucus had a ratio of 2.7. The ratio of acidic to neutral glycoproteins secreted by tracheal explants varied with duration of incubation of the trachea in culture.