| Main Title |
Genetic engineering of enhanced microbial nitrification / |
| Author |
Carsiotis, Michael. ;
Khanna, S.
|
| Other Authors |
|
| CORP Author |
Cincinnati Univ., OH. Dept. of Microbiological and Molecular Genetics.;Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab. |
| Publisher |
U.S. Environmental Protection Agency, Risk Reduction Engineering Laboratory, |
| Year Published |
1989 |
| Report Number |
EPA/600-M-89-011; EPA-R-810888 |
| Stock Number |
PB89-208334 |
| OCLC Number |
21115522 |
| Subjects |
Nitrification ;
Genetic engineering
|
| Additional Subjects |
Nitrification ;
Nitrobacter ;
Bacterial proteins ;
Genetic engineering ;
Resistance plasmids ;
Nitrobacter hamburgenesis ;
Genetic vectors ;
Transfection ;
Waste water treatment
|
| Internet Access |
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| EHAM |
EPA/600/M-89/011 |
|
Region 1 Library/Boston,MA |
05/25/2016 |
| EJBD ARCHIVE |
EPA 600-M-89-011 |
In Binder |
Headquarters Library/Washington,DC |
02/15/2017 |
| EJBD |
EPA 600-M-89-011 |
c.1 |
Headquarters Library/Washington,DC |
11/01/2013 |
| ELBD RPS |
EPA 600-M-89-011 |
repository copy |
AWBERC Library/Cincinnati,OH |
10/17/2014 |
| NTIS |
PB89-208334 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
6, [1] pages : illustrations ; 28 cm. |
| Abstract |
Experiments were conducted to introduce genetic information in the form of antibiotic or mercuric ion resistance genes into Nitrobacter hamburgenesis strain X14. The resistance genes were either stable components of broad host range plasmids or transposable genes on plasmids presumably unable to replicate in strain X14. Four methods for plasmid transformation as well as conjugation with various donor strains of Escherichia coli failed. The leuB gene containing DNA was restriction-mapped and the 1.3 kilobase pair gene was subcloned into a vector suitable for use in DNA sequencing. To date, a tentative sequence comprising about 1300 bases has been obtained. Although the primary goal of developing a procedure for introducing genetic material into a nitrifying organism has not yet been achieved, the results achieved have produced useful information on the genomic organization of Nitrobacter as well as a plasmid-borne library of genes from that organism. Future experiments can be made with this library in order to provide additional basic information on Nitrobacter's genome. |
| Notes |
Caption title. Shipping list no.: 89-379-P. "June 1989." Includes bibliographical references (pages 6-7). "EPA/600-M-89-011." |
