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Main Title Structure and Comparative Analysis of the Genes Encoding Component C of Methyl Coenzyme M Reductase in the Extremely Thermophilic Archaebacterium 'Methanothermus fervidus'.
Author Weil, C. F. ; Cram, D. S. ; Sherf, B. A. ; Reeve, J. N. ;
CORP Author Ohio State Univ., Columbus. Dept. of Microbiology.;Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab.;Office of Naval Research, Arlington, VA.;Department of Energy, Washington, DC.
Publisher c1988
Year Published 1988
Report Number EPA-R-812774 ;N00014-86-K-0211; EPA/600/J-88/369;
Stock Number PB90-100496
Additional Subjects Peptides ; Micrococcaceae ; Deoxyribonucleic acids ; Reprints ; Methanothermus fervidus ; Archaebacteria ; Genetic transcription ; Methyl coenzyme M reductase ; Molecular cloning ; Base sequence ; Amino acid sequence ; Codon
Library Call Number Additional Info Location Last
NTIS  PB90-100496 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 12p
A 6-kilobase-pair region of the genome of the extremely thermophilic archaebacterium Methanothermus fervidus which encodes the alpha, beta, and gamma subunit polypeptides of component C of methyl coenzyme M reductase was cloned and sequenced. Genes encoding the beta (mcrB) and gamma (mcrG) subunits were separated by two open reading frames designated merC and merD which encode unknown gene products. The M. fervidus genes were preceded by ribosome-binding sites, separated by short A + T-rich intergenic regions, contained unexpectedly few NNC codons, and exhibited inflexible codon usage at some locations. Sites of transcription initiation and termination flanking the mcrBDCGA cluster of genes in M. fervidus were identified. The sequences of the genes, the encoded polypeptides, and transcription regulatory signals in M. fervidus were compared with the functionally equivalent sequences from two mesophilic methanogens (Methanococcus vannielii and Methanosarcina barkeri) and from a moderate thermophile (Methanobacterium thermoautotrophicum Marburg).