Record Display for the EPA National Library Catalog


Main Title Archaebacterial RNA Polymerase Binding Site and Transcription Initiation of the hisA Gene in 'Methanococcus vannielii'.
Author Brown, J. W. ; Thomm, M. ; Beckler, G. S. ; Frey, G. ; Stetter, K. O. ;
CORP Author Ohio State Univ., Columbus. Dept. of Microbiology. ;Regensburg Univ. (Germany, F.R.). Lehrstuhl fuer Mikrobiologie.;Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab.;Department of Energy, Washington, DC.
Publisher c1988
Year Published 1988
Report Number EPA-R-810340; EPA/600/J-88/368;
Stock Number PB90-100488
Additional Subjects Reprints ; Archaebacteria ; Methanococcus vannielii ; RNA polymerases ; Genetic transcription ; Binding sites ; Promotor regions(Genetics) ; Bacterial genes ; Restriction mapping ; DNA probes ; Polyacrylamide gel electrophoresis
Library Call Number Additional Info Location Last
NTIS  PB90-100488 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 17p
Transcription initiation of the hisA gene in vivo in the archaebacterium Methanococcus vannieliim as determined by nuclease S1 and primer extension analyses occurs 73 base pairs (bp) upstream of the translation initiation site. Binding of M vannielii RNA polymerase protects 43 bp of DNA, from 35 bp upstream (-35) to 8 bp downstream (+8) of the hisA mRNA initiation site, from digestion by DNase I and exonuclease III. An A+T rich region, with a sequence which conforms to the consensus sequence for promoters of stable RNA-encoding genes in methanogens, is found at the same location (-25) upstream of the polypeptide-encoding hisA gene. It appears therefore that a TATA-like sequence is also an element of promoters which direct transcription of polypeptide-encoding genes in this archaebacterium. (Copyright (c) IRL Press Limited, Oxford, England.)