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Main Title Nucleotide Sequencing and Transcriptional Mapping of the Genes Encoding Biphenyl Dioxygenase, a Multicomponent Polychlorinated-Biphenyl-Degrading Enzyme in 'Pseudomonas' Strain LB400.
Author Erickson, B. D. ; Mondello, F. J. ;
CORP Author General Electric Corporate Research and Development, Schenectady, NY.;Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab.
Publisher c1992
Year Published 1992
Report Number EPA/600/J-93/299;
Stock Number PB93-222909
Additional Subjects DNA sequence analysis ; Genetic transcription ; Pseudomonas ; Polychlorobiphenyl compounds ; Biodeterioration ; Deoxyribonucleic acids ; Amino acid sequence homology ; Reprints ; Biphenyl dioxygenase
Library Call Number Additional Info Location Last
NTIS  PB93-222909 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 12p
The DNA region encoding biphenyl dioxygenase, the first enzyme in the biphenyl-polychlorinated biphenyl degradation pathway of Pseudomonas species strain LB400, was sequenced. Six open reading frames were identified, four of which are homologous to the components of toluene dioxygenase from Pseudomonas putida F1 and have been named bphA, bphE, bphF, and bphG. From this comparison, biphenyl dioxygenase was found to be a multicomponent enzyme containing a two-subunit iron-sulfur protein, a ferredoxin, and a reductase. Comparison of the large subunit of the iron-sulfur protein and the ferredoxin with other multicomponent dioxygenases identified amino acid sequences similar to Rieske iron-sulfur proteins from binding a (2Fe-2S) cluster. Sequences have also been identified in the reductase component that match the consensus sequence for FAD or NAD binding. Transcription of the biphenyl dioxygenase region was examined, and three transcription initiation sites were identified. Transcription initiating at the site furthest upstream is greatly increased when the LB400 cells are grown on biphenyl as the sole carbon source. (Copyright (c) 1992, American Society for Microbiology.)