Record Display for the EPA National Library Catalog


Main Title Gene Probe for PO Messenger RNA Used to Index Acrylamide Toxic Neuropathy in Rats.
Author Veronesi, B. ; Jones, K. ; Gupta, S. ; Pringle, J. ; Mezei., C. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. Neurotoxicology Div. ;Dalhousie Univ., Halifax (Nova Scotia). Dept. of Biochemistry.
Publisher c1991
Year Published 1991
Report Number EPA/600/J-92/023;
Stock Number PB92-143957
Additional Subjects Toxicity ; Acrylamides ; Messenger RNA ; DNA probes ; Nervous system ; Immunohistochemistry ; Myelin proteins ; Glycoproteins ; Northern blotting ; Electron microscopy ; Genetic transcription ; Axons ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB92-143957 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 20p
Cumulative exposure to the neurotoxicant acrylamide produces axonal damage in the distal ends of both central (CNS) and peripheral (PNS) nerve fibers and subsequent hind-limb paralysis. The messenger RNA which codes for the PNS myelin glycoprotein PO(PO-mRNA) was used to monitor this toxic neuropathy in Sprague Dawley rats prior to, concurrent with, and subsequent to, ultrastructurally and immunocytochemically defined nerve damage. Rats were dosed every other day with acrylamide (50 mg/kg, IP) and sampled intermittently throughout a 4 week exposure period. Slot blot and Northern gel analyses of the proximal and distal sciatic nerve were used to determine a quantitated measure of PO-mRNA. Results suggest that changes in neural specific mRNAs are sensitive to neurotoxic damage and can be used to monitor the pathogenesis of nerve degeneration.