Record Display for the EPA National Library Catalog


Main Title Cell Growth in Plant Cultures: An Interpretation of the Influence of Initial Weight in Cadmium and Copper Toxicity Tests.
Author Shirazi, M. A. ; Robideaux, M. L. ; Kapustka, L. A. ; Wagner, J. J. ; Reporter, M. C. ;
CORP Author Corvallis Environmental Research Lab., OR. ;ManTech Environmental Technology, Inc., Corvallis, OR. Environmental Research Lab. ;Ecological Planning and Toxicology, Inc., Corvallis, OR. ;Battelle Pacific Northwest Labs., Richland, WA. ;Oregon State Univ., Corvallis. Dept. of Botany and Plant Physiology.
Publisher c1994
Year Published 1994
Report Number EPA/600/J-94/468;
Stock Number PB95-137303
Additional Subjects Toxicity ; Cultures(Biology) ; Plant cells ; Tissue culture ; Cadmium ; Copper ; Dosage ; Growth ; Mathematical models ; Weight gain ; Statistical analysis ; Heavy metals ; Tests ; Reprints ; Medicago sativa ; Green Mandevilla pohliana ; Non-green Mandevilla pohliana
Library Call Number Additional Info Location Last
NTIS  PB95-137303 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 9p
The authors present an approach for conducting and interpreting results of newly established plant cell culture in toxicity studies. Extended culturing produces uniform suspension and facilities sampling. Primary (new) cultures are more representative of all responses of their plant species but produce nonuniform suspension due to clumping and clumping presents unavoidable sampling difficulties. Evaluations were made of the response of plant cell suspension in primary cultures subjected to metal stress in three different experiments. Sampling and data interpretation were examined in detail, using mathematical models. The model revealed stimulation of cell growth when subjected to low concentration of copper and no-growth stimulation with low or moderate concentration of cadmium. This finding resolves some of the confusion in the literature relative to the effect of low doses of cadmium.