Record Display for the EPA National Library Catalog


Main Title Isolation of the Alkane Inducible Cytochrome P450 (P450alk) Gene from the Yeast 'Candida tropicalis'.
Author Sanglard, D. ; Chen, C. ; Loper, J. C. ;
CORP Author Cincinnati Univ. Medical Center, OH. Dept. of Microbiology and Molecular Genetics.;Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab.
Publisher c1987
Year Published 1987
Report Number EPA-CR-810605; EPA/600/J-93/306;
Stock Number PB93-229284
Additional Subjects Cytochrome P-450 ; Alkanes ; Enzyme induction ; Saccharomyces cerevisiae ; Fungal DNA ; Genetic transcription ; Molecular sequence data ; Messenger RNA ; Reprints ; Candida tropicalis
Library Call Number Additional Info Location Last
NTIS  PB93-229284 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 9p
The gene for the alkane-inducible cytochrome P450, P450alk, has been isolated from the yeast Candida tropicalis by immunoscreening a lambda gt11 library. Isolation of the gene has been identified on the basis of its inducibility and partial DNA sequence. Transcripts of this gene were induced by alkane to levels 500 to 1000 fold over those detected in glucose-grown cells. The nucleotide sequence of the 3' portion of this gene revealed a coding sequence for the heme binding segment characteristic of the P450 gene family. (Copyright (c) 1987 Academic Press, Inc.)