Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Evaluation of a new fluorometric technique that uses highly selective interference filters for measuring chlorophyll a in the presence of chlorophyll b and pheopigments : summary report /
Author Arar, Elizabeth J. ; Arar, E. J.
CORP Author Environmental Monitoring Systems Lab., Cincinnati, OH. Chemistry Research Div.
Publisher U.S. Environmental Protection Agency, Environmental Monitoring Systems Laboratory, Office of Research and Development,
Year Published 1994
Report Number EPA/600/R-94/150;
Stock Number PB94-210622
OCLC Number 42801752
Subjects Chlorophyll.
Additional Subjects Fluorometers ; Chlorophylls ; Test methods ; Performance evaluation ; Plant pigments ; Filters ; Parameters ; Optical measuring instruments ; Spectrophotometry ; Fluorescence ; Chlorophyll a ; Chlorophyll b ; Phenophytin a
Internet Access
Description Access URL
Library Call Number Additional Info Location Last
ELBD  EPA 600-R-94-150 AWBERC Library/Cincinnati,OH 09/08/2000
ESAD  EPA 600-R-94-150 Region 10 Library/Seattle,WA 11/19/1999
NTIS  PB94-210622 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 04/04/2019
Collation vii, 24 p. : ill. ; 28 cm.
A new fluorometric technique was compared to conventional fluorometry with and without pheophytin a (pheo a) correction and to spectrophotometry using Lorenzen's modified monochromatic equations and Jeffrey and Humphrey's trichromatic equation to calculate chlorophyll a (chl a). The new technique uses excitation and emission filters that are highly selective for chl a, eliminating the interference caused by accessory pigments and pheopigment degradation products. Four method parameters were evaluated using the new technique, conventional fluorometry and spectrophotometry. They were (1) sensitivity, (2) linear dynamic range, (3) precision, and (4) accuracy.
Bibliography: p. 9.