Main Title |
Evaluation of a new fluorometric technique that uses highly selective interference filters for measuring chlorophyll a in the presence of chlorophyll b and pheopigments : summary report / |
Author |
Arar, Elizabeth J. ;
Arar, E. J.
|
CORP Author |
Environmental Monitoring Systems Lab., Cincinnati, OH. Chemistry Research Div. |
Publisher |
U.S. Environmental Protection Agency, Environmental Monitoring Systems Laboratory, Office of Research and Development, |
Year Published |
1994 |
Report Number |
EPA/600/R-94/150; |
Stock Number |
PB94-210622 |
OCLC Number |
42801752 |
Subjects |
Chlorophyll
|
Additional Subjects |
Fluorometers ;
Chlorophylls ;
Test methods ;
Performance evaluation ;
Plant pigments ;
Filters ;
Parameters ;
Optical measuring instruments ;
Spectrophotometry ;
Fluorescence ;
Chlorophyll a ;
Chlorophyll b ;
Phenophytin a
|
Internet Access |
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
ELBD |
EPA 600-R-94-150 |
|
AWBERC Library/Cincinnati,OH |
09/08/2000 |
ESAD |
EPA 600-R-94-150 |
|
Region 10 Library/Seattle,WA |
11/19/1999 |
NTIS |
PB94-210622 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
vii, 24 p. : ill. ; 28 cm. |
Abstract |
A new fluorometric technique was compared to conventional fluorometry with and without pheophytin a (pheo a) correction and to spectrophotometry using Lorenzen's modified monochromatic equations and Jeffrey and Humphrey's trichromatic equation to calculate chlorophyll a (chl a). The new technique uses excitation and emission filters that are highly selective for chl a, eliminating the interference caused by accessory pigments and pheopigment degradation products. Four method parameters were evaluated using the new technique, conventional fluorometry and spectrophotometry. They were (1) sensitivity, (2) linear dynamic range, (3) precision, and (4) accuracy. |
Notes |
Bibliography: p. 9. |