Record Display for the EPA National Library CatalogRECORD NUMBER: 3 OF 10
|Main Title||Flow Cytometric Analysis of the Mechanism of Methylmercury Cytotoxicity.|
|Author||Zucker, R. M. ; Elstein, K. H. ; Easterling, R. E. ; Massaro, E. J. ;|
|CORP Author||Health Effects Research Lab., Research Triangle Park, NC. Developmental Toxicology Div. ;NSI Technology Services Corp., Research Triangle Park, NC.|
|Additional Subjects||Methylmercury compounds ; Toxicity ; Fluorescence spectrometry ; Cell survival ; Flow cytometry ; Dose-response relationships ; Cell membrane ; Erythro leukemia ; Culture tumor cells ; Cell cycle ; Chromosomes ; Mitosis ; Computerized simulation ; Reprints ;|
The target and degree of methylmercury cytotoxicity are dose-dependent. Following 6 h exposure to 2.5 - 7.5 microMolar, methylmercury (MeHg) progressively inhibits DNA synthesis and induces chromosomal damage in murine erythroleukemic cells. However, following exposure to 10 - 50 microMolar MeHg, the plasma membrane/cytoplasm complex is grossly perturbed, cell cycle progression is blocked, and chromosomes appear in ring formations. These findings, together with those previously observed following organotin exposure, also suggest that severe and nonspecific toxicity may be a common endpoint of exposure to high levels of organometals.