Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Improved Method for Recovery of Enteric Viruses from Wastewater Sludges.
Author Hurst, C. J. ; Goyke, T. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1986
Report Number EPA/600/J-86/254;
Stock Number PB87-171070
Additional Subjects Enteroviruses ; Recovery ; Sludge ; Waste water ; Optimization ; Adsorption ; Extraction ; Beef ; Concentration(Composition) ; Flocculating ; pH ; Aluminum chloride ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB87-171070 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/21/1988
Collation 6p
Various parameters involved in recovering indigenous enteric viruses from wastewater sludges aided by buffered beef extract elution and subsequent organic flocculation concentration were examined. Conditions were optimized to yield an overall effective method for use in environmental virus occurrence studies. Initial adsorption of viruses to the sludge solids was best accomplished by adjustment of the samples to pH 3.5 followed by supplementation with aluminum chloride. Elution of the solids-adsorbed viruses was achieved by agitating the solids for 30 minutes in five equivalent volumes of pH 7.4, buffered 10% beef extract solution. Concentration of viruses from the eluants was accomplished by organic flocculation at pH 3.5