1. Introduction -- 2. Basic Concepts and the Control of Separation -- 3. Equipment -- 4. Detection -- 5. Column -- 6. Reversed-Phase Chromatography for Neutral Samples -- 7. Ionic Samples : Reversed-Phase, Ion-Pair, and Ion-Exchange Chromatography -- 8. Normal-Phase Chromatography -- 9. Gradient Elution -- 10. Computer-Assisted Method Development -- 11. Qualitative and Quantitative Analysis -- 12. Method Validation / Michael Swartz -- 13. Biochemical and Synthetic Polymer Separations / Timothy Wehr, Carl Scandella, Peter Schoenmakers -- 14. Enantiomer Separations / Michael Lämmerhofer, Nobert M. Maier, Wolfgang Linder -- 15. Preparative Separations / Geoff Cox -- 16. Sample Preparation / Ronald Majors -- 17. Troubleshooting -- Appendix I : Properties of HPLC Solvents -- Appendix II : Preparing Buffered Mobile Phases. "High-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and/or purify virtually any sample. Snyder and Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier reference to HPLC. This Third Edition, with John Dolan as added coauthor, addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve problems that were troublesome in the past, and the application of HPLC for new kinds of samples." "This carefully considered Third Edition maintains the strengths of the previous edition while significantly modifying its organization in light of recent research and experience. The text begins by introducing the reader to HPLC, its use in relation to other modern separation techniques, and its history, then leads into such specific topics." "Designed to fulfill the needs of the full range of HPLC users, from novices to experts, Introduction to Modern Liquid Chromatography, Third Edition offers the most up-to-date, comprehensive, and accessible survey of HPLC methods and applications available."--Jacket.