| Main Title |
Microinjection of fish embryos as a laboratory assay for chemical carcinogens / |
| Author |
Grizzle, John M. ;
Grizzle, J. M. ;
Putnam, M. R.
|
| Other Authors |
|
| CORP Author |
Auburn Univ., AL.;Environmental Research Lab., Gulf Breeze, FL. |
| Publisher |
Environmental Research Laboratory, Office of Research and Development, U.S. Environmental Protection Agency, |
| Year Published |
1987 |
| Report Number |
EPA/600/3-87/032; PB88124623 |
| Stock Number |
PB88-124623 |
| OCLC Number |
18210492 |
| Subjects |
Fishes--Effect of water pollution on
|
| Additional Subjects |
Carcinogens ;
Fishes ;
Injections(Medicine) ;
Embryos ;
Laboratory animals ;
Exposure ;
Chemical tests ;
Neoplasms ;
Medical research ;
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| EKCD |
EPA/600/3-87/032 |
|
CEMM/GEMMD Library/Gulf Breeze,FL |
07/17/2018 |
| NTIS |
PB88-124623 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
vii, 30 pages ; 28 cm |
| Abstract |
Techniques were developed for microinjection of chemicals into fish embryos for use in carcinogenicity assays. Eggs of the following were injected: gulf killifish (Fundulus grandis), sheepshead minnow (Cyprinodon variegatus), rivulus (Rivulus ocellatus), inland silverside (Menidia beryllina), gulf toadfish (Opsanus beta), and channel catfish (Ictalurus punctatus) Chemicals injected into eggs were diethylnitrosamine (DEN), aflatoxin B1 (AFB), N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), and trichloroethylene (TCE). Quantification of carcinogen dose in the egg immediately after injection indicated that variation of the dose retained was a major problem. Improvements in procedures resulted in increased mean percentage of the dose remaining in the egg, but variation between eggs remained high. |
| Notes |
"November 1987." Includes bibliographical references (pages 29-30). Contract no. CR813425-01. Microfiche. |
