Record Display for the EPA National Library Catalog

RECORD NUMBER: 260 OF 487

Main Title Histochemical and cytophotometric assay of acid stress in freshwater fish /
Author Anthony, Adam.
Other Authors
Author Title of a Work
Cooper, Edwin.
Mitchell, Robert B.,
Neff, William H.,
Therrien, Chester D.
Anthony, Adam.
CORP Author Pennsylvania State University. Dept. of Biology.
Publisher United States Environmental Protection Agency, Water Quality Office,
Year Published 1971
Report Number EPA18050-DXJ-05/71; EPA 841-R-71-001; EPA 620-R-41-003
OCLC Number 02695299
Subjects Acid pollution of rivers, lakes, etc ; Fishes--Effect of water pollution on ; Water quality bioassay ; Fishes--Diseases
Additional Subjects Acid pollution of rivers, lakes, etc ; Fishes, Effect of water pollution on ; Water quality bioassay ; Fishes--Diseases
Internet Access
Description Access URL
https://nepis.epa.gov/Exe/ZyPDF.cgi?Dockey=9100ULJG.PDF
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
EJED  EPA 620-R-71-003 OCSPP Chemical Library/Washington,DC 01/12/2018
ELBD ARCHIVE EPA 620-R-71-003 Received from HQ AWBERC Library/Cincinnati,OH 10/04/2023
ELBD  EPA 841-R-71-001 AWBERC Library/Cincinnati,OH 10/28/2011
Collation x, 113 pages : illustrations, figures, tables ; 28 cm
Notes
"Report date May 1971." Includes bibliographical references (pages 85-92).
Contents Notes
The feasibility of using histochemical and histopathological changes in brook trout, longnose dace and fathead minnows as bioindicators of acid pollution was investigated. Laboratory studies entailed using a gravity flow diluter system. Field studies involved using net traps in polluted streams. Exposure durations were 4-5 days and 28-30 days. Histochemical and cytophotometric analyses were made of gills, Stannius corpuscle, blood, spleen, kidney and liver. The primary mode of acid toxicant action is gill damage which results in impaired respiratory, excretory and liver functions. Short term indices of acid stress include: colloidal iron and aminosalicylic acids (PAS) staining of gills and renal Stannius corpuscles. A useful bioindicator of prolonged acid exposure is decreased azure B-RNA staining of liver cells; this assesses the extent of liver impairment and reflects a reduced tolerance of fish to other toxicants.