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Main Title A continuous flow system using fish and amphibian eggs for bioassay determinations on embryonic mortality and teratogenesis /
Author Birge, Wesley J. ; Black., Jeffrey A.
Other Authors
Author Title of a Work
Black, Jeffrey A.,
CORP Author United States. Environmental Protection Agency. Office of Toxic Substances.; Kentucky.
Publisher Environmental Protection Agency, Office of Toxic Substances,
Year Published 1977
Report Number EPA 560/5-77-002; EPA-68-01-4321
Stock Number PB-285 191
OCLC Number 02995378
ISBN pbk.
Subjects Biological assay ; Fishes--Eggs--Hatchability ; Fishes--Embryology ; Amphibians
Additional Subjects Biological assay ; Fishes--Eggs--Hatchability ; Embryology--Fishes ; Amphibians ; Toxicology ; Bioassay ; Concentration(Composition) ; Contaminants ; Embryos ; Water pollution ; Pathology ; Mutations ; Eggs ; Exposure ; Physicochemical properties ; Mortality ; Tables(Data) ; Experimental data ; Survival ; Lethal dosage ; Toxic substances ; Teratogenesis ; Water pollution effects(Animals) ; Trace amounts
Internet Access
Description Access URL
Library Call Number Additional Info Location Last
EJBD ARCHIVE EPA 560-5-77-002 Headquarters Library/Washington,DC 12/23/2019
NTIS  PB-285 191 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation ii, 59, [1] pages : illustrations ; 28 cm.
A procedure is described for continuous flow bioassays on sensitive developmental stages of fish and other aquatic species. Environmental toxicants may be screened for teratogenic and other embryopathic effects (e.g., mortality, locomotor impairment) on eggs, embryos, and early juvenile stages. Toxicant concentration may be regulated down to 1.0-0.1 ppb for an exposure period extending from fertilization through 4-7 days posthatching. Bioassays may be performed with either natural or synthetic water, and standard physicochemical parameters (e.g., temperature, water hardness, pH) can be manipulated independently to determine effects on toxicity. The procedure is suitable for use with a wide range of fish and amphibian species, including rainbow trout, channel catfish, largemouth bass, leopard frog and others. Concerning the toxic effects of many environmental trace contaminants, embryonic and early juvenile stages constitute the critical sensitive links in the life cycles of many aquatic animal species. Reproductive potential of aquatic animals may be severely restricted or abolished by trace levels of toxicants which are harmless or sublethal to most adult organisms.
Final technical report, Apr. 1977. Performing organization: University of Kentucky. EPA Contract no. 68-01-4321. Issued Apr. 1977. Includes bibliographical references (pages 56-59).