Record Display for the EPA National Library CatalogRECORD NUMBER: 6 OF 7
|Main Title||New Method for Excystation of Giardia.|
|Author||Sauch, J. F. ;|
|CORP Author||Health Effects Research Lab., Research Triangle Park, NC.|
|Additional Subjects||Giardia ; Cysts ; In vitro analysis ; Peptones ; Agents ; Trypsin ; Enzymes ; Microbiology ; Excystation ; Antigenic analysis ; Protease peptone ; Phytone peptone ; Hanks Balanced Salt Solution(HBSS) ; Giardia lamblia ; Giardia muris ; Electrophoretic analysis|
In vitro excystation of Giardia is used to estimate cyst viability and may also be used to obtain trophozoites and cyst walls for analysis. Recently published excystation procedures include the use of trypsin, serum or bile salts in the excystation step. Trypsin, serum or bile salts in the excystation medium was replaced by proteose peptone for G. muris and phytone peptone for G. lamblia. Comparison between this method utilizing peptones and that of Schaefer utilizing trypsin showed no significant differences in percent excystation for both G. muris and G. lamblia.