||Norepinephrine Decreases EGF (Epidermal Growth Factor) Binding in Primary Rat Hepatocyte Cultures.
Cruise, J. L. ;
Cotecchia, S. ;
Michalopoulos, G. ;
||Duke Univ. Medical Center, Durham, NC. Dept. of Pathology.;Health Effects Research Lab., Research Triangle Park, NC.;National Institutes of Health, Bethesda, MD.
Laboratory animals ;
Epidermal growth factor-Urogastrone ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
Norepinephrine (NE) produced a dose-dependent inhibition of 125I-epidermal growth factor (EGF) binding to adult rat hepatocytes in primary culture. The effect was maximal after 1 hour of incubation with NE and could be blocked by the presence of an alpha 1 -specific adrenergic receptor antagonist. The inhibition of binding correlates with the ability of NE to enhance hepatocyte DNA synthesis in the presence of EGF and appears to be mediated by a reduction of EGF receptor number, without a significant change in receptor affinity. (Copyright (c) 1986 Alan R. Liss, Inc.)