Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Mutagenicity of Nitrite-Treated Aqueous Extract of 'Piper betle'; L.
Author Chen, H. C. ; Chang, Y. S. ; Lee, T. C. ;
CORP Author Rhode Island Univ., Kingston. ;National Taiwan Coll. of Marine Science and Technology, Keelung.;Environmental Research Lab., Narragansett, RI.
Year Published 1985
Report Number EPA/600/D-85/126;
Stock Number PB85-220408
Additional Subjects Asia ; Nitroso compounds ; Toxicity ; Betel quid ; Carcinomas ; Chewing ; Mastication ; Mutagens ; Piper betle ; Salmonella typhimurium ; Piperidine/N-nitroso ; Pyrrolidine/N-nitroso ; Morpholine/N-nitroso ; Epidemiologic methods
Library Call Number Additional Info Location Last
NTIS  PB85-220408 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/21/1988
Collation 10p
Betel quid is chewed as a masticatory material by people in certain areas of Asia. The quid chewing has been related to oral cancer by epidemiological study. The mutagenic components in the aqueous extracts of betel quid ingredients were studied. Only nitrite-treated aqueous extract of Piper betle L fruits, leaves or rhizoma were demonstrated to exhibit a mutagenic response, using Salmonella typhimurium strains TA100 and TA1535 in the Ames test. When the aqueous extract of the fruit was nitrosated, the greatest number of mutagenic substances were formed at pH 3. The formation of mutagens was enhanced by increasing the temperature from 5 to 95 deg. C. Maximum production of the mutagens occurred within 15 min when nitrosation was conducted at 35 deg. C. The mutagenic components in nitrite-treated aqueous extract of Piper betle L fruit were found to be N-nitrosopiperidine, N-nitrosopyrrolidine, N-nitrosomorpholine, and other compounds, as determined by gas chromatography-thermal energy analyzer.