Record Display for the EPA National Library Catalog


Main Title Neutrophil Myeloperoxidase Destruction by Ultraviolet Irradiation.
Author Hanker, J. ; Giammara, B. ; Strauss, G. ;
CORP Author North Carolina Univ. at Chapel Hill. ;Louisville Univ., KY.;Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div.
Publisher c1988
Year Published 1988
Report Number EPA/600/J-88/508;
Stock Number PB90-216011
Additional Subjects Neutrophils ; Ultraviolet radiation ; Eosinophils ; Microscopy ; Blood ; Psoriasis ; Reprints ; Myeloperoxidase ; PUVA therapy ; Scanning electron microscopy
Library Call Number Additional Info Location Last
NTIS  PB90-216011 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 4p
The peroxidase activity of enriched leukocyte preparations on coverslips was determined cytochemically with a newly developed method. The techniques utilizes diaminobenzidine medium and cupric nitrate intensification and is suitable for analysis with light microscopy, SEM, and TEM. Blood specimens from control individuals were studied with and without in vitro UV irradiation and compared with those from psoriasis patients exposed therapeutically to various types of UV in phototherapy. All UV irradiated samples showed diminished neutrophil myeloperoxidase (MP) activity although that of the principal eosinophil peroxidase was unaffected. The SEMs supported the contention that decreased neutrophil MP activity might be related to UV induced degranulation. It is believed to be possible, eventually, to equate the observed MP degranulation effect after UV irradiation with diminished ability to fight bacterial infections. (Copyright (c) 1988 by San Francisco Press, Inc.)