| Contents Notes |
Basic methods -- Laboratory requirements and media -- General cell culture techniques -- Specialized techniques -- Metabolism -- Genetics, hybridization, and transformation -- Virus preparation -- Specific cell lines. Physical aspects of a tissue culture laboratory / William H.J. Douglas, Robert T. Dell'Orco -- Detection of contamination / Gerard J. McGarrity -- Preservation, storage, and shipment / Lewis L. Coriell -- Safety considerations in the cell culture laboratory / W. Emmett Barkley -- Media and growth requirements / Richard G. Ham, Wallace L. McKeehan -- The growth of cells in serum-free hormone-supplemented media / Jane Bottenstein, Izumi Hayashi, Sharon Hutchings, Hideo Masui, Jennie Mather, Don B. McClure, Sugayuki Ohasa, Angie Rizzino, Gordon Sato, Ginette Serrero, Richard Wolfe, Reen Wu -- Use of antibiotics in cell culture media / D. Perlman -- Laboratory management of cell cultures / Ken Wolf -- Dispersion and disruption of tissues / Mark M. Bashor -- Monolayer culture techniques / James A. McAteer, William H.J. Douglas -- Measurement of growth and viability of cells in culture / Manford K. Patterson Jr. -- Cloning / Lola C.M. Reid -- Cell culture characterization: Monitoring for cell identification / Ward D. Peterson Jr., William F. Simpson, Bharati Hukku -- Tissue Culture on Artificial Capillaries / P.M. Gullino, R.A. Knazek -- Microcarrier culture: A homogeneous environment for studies of cellular biochemistry / William G. Thilly, David W. Levine -- Mass culture of mammalian cells / William F. McLimans -- Propagation and scaling-up of suspension cultures / Ronald T. Acton, Paul A. Barstad, Robert K. Zwerner -- Harvesting the products of cell growth / Robert K. Zwerner, Kim S. Wise, Ronald T. Action -- Cell cycle analysis by flow cytometry / J.W. Gray, P. Coffino -- Cell synchronization / Tsukasa Ashihara, Renato Baserga -- New Techniques for Culturing Differential Cells: Reconstituted basement membrane rafts / Lola M. Reid, Marcos Rojkind -- Autoradiography / Gretchen H. Stein, Rosalind Yanishevsky -- Induction and production of interferon / Robert M. Friedman -- Evaluation of chemical carcinogenicity by in vitro neoplastic transformation / Noèel Bouck, Giampiero di Mayorca -- Independent control of the local environment of somas and neurites / Robert B. Campenot -- Mutant isolation / Larry H. Thompson -- Karyotyping / Ronald G. Worton, Catherine Duff -- Cell fusion / Roger H. Kennett -- Fusion of higher plant protoplasts / Albert W. Ruesink -- Cell transformation / Ira Pastan -- Use of nude mice for tumorigenicity testing and mass propagation / Seung-il Shin -- Biological techniques for avian sarcoma viruses / Eric Hunter -- Large-scale growth of Rous sarcoma virus / Ralph E. Smith -- Preparation of Simian Virus 40 and its DNA / George Khoury, Ching-Ju Lai -- Purification and assay of murine leukemia viruses / Charles J. Sherr, George J. Todaro -- Human adenoviruses: Growth, purification, and transfection assay / Maurice Green, William S.M. Wold -- Sources of stable cell lines / Gerard J. McGarrity -- Isolation of fibroblasts from patients / William S. Sly, Jeffrey Grubb -- Cell lines from invertebrates / W. Fred Hink -- Cold-blooded vertebrate cell and tissue culture / Ken Wolf -- Plant cell lines / John F. Reynolds, Toshio Murashige -- Lymphocytes as resting cells / Shelby L. Berger -- Macrophages / Dolph O. Adams -- Mouse erythroleukemia cells / T.V. Gopalakrishnan, W. French Anderson -- Skeletal myoblasts in culture / Irwin R. Konigsberg -- Clonal strains of hormone-producing pituitary cells / Armen H. Tashjian Jr. -- Liver cells / H.L. Leffert, K.S. Koch, T. Moran, M. Williams -- Liver cells (HTC) / E. Brad Thompson -- An established but differentiated kidney epithelial cell line (MDCK) / Mary Taub, Milton H. Saier Jr. -- Large-scale preparation of chondrocytes / Michael Klagsbrun -- Melanoma cells / John Pawelek -- Adrenocortical Y1 cells / Bernard P. Schimmer -- Long-term culture of dissociated sympathetic neurons / Edward Hawrot, Paul H. Patterson -- Neuronal cells from rodent neoplasma / David Schubert, William Carlisle. |
