Translation of Methoden der enzymatischen Analyse. Includes bibliographical references and index.

Section A. General introduction -- I. The importance of enzymatic analysis -- 1. In biochemistry -- 2. In medicine -- 3. In foodstuff chemistry -- 4. In botany and agricultural chemistry -- 5. In microbiology -- II. Principles of enzymatic analysis -- 1. Introduction -- 2. Reaction kinetics -- 3. Determination of concentration of metabolites (end-point methods) -- 4. Determination of enzyme activities -- 5. Determination of concentrations by kinetic methods -- 6. Kinetics of "enzymatic cycling" -- 7. Visualization of NAD(P)-dependent reactions -- 8. Determination of Michaelis constants and inhibitor constants -- III. Experimental techniques -- 1. Handling of biochemical reagents and samples -- 2. Methods of measurement and instruments used -- 3. Evaluation of experimental results -- IV. Statistical analysis, control and assessment of experimental results -- 1. Introduction -- 2. Statistical concepts and methods -- 3. Quality control -- 4. Assessment of results -- V. Cell and tissue disintegration -- 1. General -- 2. Methods for animal tissue and micro-organisms -- 3. Methods for plant tissues -- Section B. Biochemical reagents -- I. Reagents for enzymatic analysis -- 1. Nomenclature and standardization -- 2. Quality requirements -- 3. Suppliers of biochemical reagents -- II. Enzymes as biochemical reagents -- III. Coenzymes, metabolites and other biochemical reagents -- IV. Complete reagent kits -- 1. For determination of metabolites -- 2. For determination of enzyme activities -- 3. Main manufacturers and suppliers -- Section D. Methods for determination of metabolites -- I. Carbohydrate metabolites -- 1. Poly-, oligo- and disaccharides -- 2. Monosaccharides and derivatives -- 3. Three carbon compounds -- 4. Two and one carbon compounds -- II. Citric acid cycle metabolites -- 1. Citrate -- 2. Isocitrate -- 3. 2-oxyglutarate -- 4. L-(-)-malate -- 5. L-malate and fumarate -- 6. Oxaloacetate -- 7. Succinate -- 8. Maleate -- III. Proteins, peptides and amino acids -- 1. Proteins and peptides -- 2. Amino acids, amines and amides -- IV. Fatty acid metabolites, lipids and steroids -- 1. Polyunsaturated fatty acids -- 2. Lecithin -- 3. Acetylcholine and choline -- 4. Triglycerides and glycerol determination after alkaline hydrolysis -- 5. Triglycerides, determination after enzymatic hydrolysis -- 6. D-(-)-3-hydroxybutyrate -- 7. Acetoacetate -- 8. Triacetate and fumarylacetoacetate -- 9. Hydrolysis of steroid conjugates -- 10. 20-ketosteroids -- 11. Steroid alcohols in urine -- 12. Prostaglandins -- 13. Bile acids -- 14. Cholesterol and esterified cholesterol -- V. Nucleic acids, purines, pyrimidines, nucleosides, coenzymes and related compounds -- 1. Nucleic acids and polyribonucleotides -- 2. Purines, pyrimidines and nucleosides -- 3. Nucleotides, coenzymes and related compounds -- 4. Nucleoside diphosphate sugars and derivatives -- VI. Other substrates and effectors -- 1. Inorganic phosphate -- 2. Inorganic pyrophosphate -- 3. Inorganic peroxides -- 4. Organophosphorus and carbamate insecticides -- 5. Nitrate -- Concentrations of metabolites in animal tissues -- 1. Glycogen, glucose and glycolytic intermediates -- 2. Tricarboxylic acid cycle intermediates -- 3. Amino acids and ammonia -- 4. Pentose phosphate pathway intermediates -- 5. Coenzyme A and intermediates of lipid metabolism -- 6. Adenosine and other nucleotides -- 7. Nicotinamide-adenine dinucleotides -- 8. Creatine phosphate, creatine, inorganic pyrophosphate and phosphate.