Record Display for the EPA National Library Catalog


Main Title ELISA : theory and practice /
Author Crowther, J. R.
Publisher Humana Press,
Year Published 1995
OCLC Number 32130600
ISBN 0896032795; 9780896032798
Subjects Enzyme-linked immunosorbent assay ; Test ELISA ; Enzymimmunassay ; Enzym immunoassay ; Wetenschappelijke technieken ; ELISA--Emploi en diagnostic ; Technique immunoenzymatique ; Diagnostic immunologique ; Enzymes
Internet Access
Description Access URL
Publisher description
Library Call Number Additional Info Location Last
EHBM  QP519.9.E48E45 1995 CEMM/ACESD Library/Narragansett,RI 11/16/2022
Collation xi, 223 pages : illustrations ; 23 cm.
Includes bibliographical references and index.
Contents Notes
Basic immunology -- Basic principles of ELISA -- Stages in ELISA -- Theoretical considerations -- Direct ELISA : titration of antigen and antibody -- Indirect ELISA -- Use of antibodies on solid-phase in capture ELISA -- Competitive ELISA -- Immunochemical techniques. ELISA: Theory and Practice introduces to scientists at all levels of expertise the principles of the most commonly used assay technique known as Enzyme Linked Immunosorbent Assay. The book provides readers with full descriptions of the basic systems that make ELISA one of the most powerful techniques in science today, and also examines in detail the data obtained by ELISA and their analysis and actual manipulation. ELISA: Theory and Practice is designed not only to train novices in the science of ELISA, but also to aid investigators experienced in any of the biological sciences in performing independently assays of antibodies and antigens. Mastery of the book's contents will allow you to fully appreciate exactly how and why assays function, as well as to efficiently develop assays suitable to your own research that are both rapid and accurate.