Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Handbook of basic microtechnique /
Author Gray, Peter,
Publisher McGraw-Hill
Year Published 1964
OCLC Number 00250679
Subjects Microtechnique. ; Microscopy. ; Einfèuhrung. ; Mikrosystemtechnik.
Additional Subjects Microscope and microscopy--Technique
Library Call Number Additional Info Location Last
EJEM  QH231.G8 1964 OCSPP Chemical Library/Washington,DC 06/19/1998
Edition 3d ed.
Collation xii, 302 pages illustrations 23 cm.
Includes bibliographical references.
Contents Notes
Part 1. The microscope -- 1. Principles of microscopy -- 2. The use of the microscope -- 3. Photomicrography -- Part 2. The preparation of microscope slides -- 4. Types of microscope slides -- 5. Materials and equipment -- 6. Fixation and fixatives -- 7. Stains and staining -- 8. Dehydrating and clearing -- 9. Mounts and mountants -- 10. Making wholemounts -- 11. Making smears and squashes -- 12. Making sections -- 13. Cleaning, labeling, and storing slides -- Part 3. Specific examples of slide making -- Example 1. Preparation of a wholemount of a mite by a method of Berlese -- Example 2. Preparation of a wholemount of Pectinatella stained in Grenacher's alcoholic borax carmine -- Example 3. Preparation of a wholemount of 33-hour chick embryo, using the alum hematoxylin stain of Delafield -- Example 4. Preparation of a wholemount of a liverfluke, using the carmalum stain of Mayer -- Example 5. Smear preparation of monocystis from the seminal vesicle of an earthworm -- Example 6. Smear preparation of human blood stained by the method of Wright -- Example 7. Staining a bacterial film with crystal violet by the technique of Lillie -- Example 8. Demonstration of Gram-positive bacteria in smear preparation by the method of Gram -- Example 9. Demonstration of tubercle bacilli in sputum by the technique of Neelsen -- Example 10. Preparation of a squash of the salivary glands of Drosophila stained in LaCour's acetic orcein -- Example 11. Preparation of a transverse section of a root, using the acid fuchsin-iodine green technique of Chamberlain -- Example 12. Preparation of a transverse section of the small intestine of the frog stained with celestine blue B-eosin -- 13. Preparation of a transverse section of a stem of Aristolochia stained by the method of Johansen -- Example 14. Demonstration of spermatogenesis in the rat testis, using the iron hematoxylin stain of Heidenhain -- Example 15. Preparation of a transverse section of the tongue of a rat, using celestine blue B followed by picro acid fuchsin -- Example 16. Preparation of a transverse section of Amphioxus using the acid fuchsin-aniline blue-orange G stain of Mallory -- Example 17. Demonstration of Diplococci in the liver of a rabbit using the phloxine-methyline blue-azur II stain of Mallory -- Example 18. Preparation of a series of demonstration slides, each having six typical transverse sections of a 72-hour chick embryo, using the acid alum hematoxylin stain of Ehrlich -- Part 4. Recommended techniques -- Animal organs -- Animal tissues -- Animal cytology -- Plant organs -- Plant tissues -- Plant cytology.