| Main Title |
Isolation of the 'Candida tropicalis' Gene for P450 Lanosterol Demethylase and Its Expression in 'Saccharomyces cerevisiae'. |
| Author |
Chen, C. ;
Turi, T. G. ;
Sanglard, D. ;
Loper, J. C. ;
|
| CORP Author |
Cincinnati Univ. Medical Center, OH. Dept. of Microbiology and Molecular Genetics.;Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab. |
| Publisher |
c1987 |
| Year Published |
1987 |
| Report Number |
EPA-CR-810605 ;EPA-CR-813366; EPA/600/J-93/303; |
| Stock Number |
PB93-229250 |
| Additional Subjects |
Cytochrome P-450 ;
Saccharomyces cerevisiae ;
Fungal genes ;
Gene expression ;
Escherichia coli ;
Fungal DNA ;
Heme ;
Drug resistance ;
Ketoconazole ;
Molecular sequence data ;
Reprints ;
Candida tropicalis
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB93-229250 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
9p |
| Abstract |
The gene for cytochrome P450 lanosterol 14alpha-demethylase (14DM) from the yeast Candida tropicalis has been isolated. This was accomplished by screening genomic libraries of strain ATCC750 in E. coli using a DNA fragment containing the yeast Saccharomyces cerevisiae 14DM gene. Identity of this gene was confirmed by (a) observing a heme binding region common to all P450s after sequencing the 3' portion of the gene, and (b) based upon tests of its expression in strains of Saccharomyces cerevisiae. (Copyright (c) 1987 Academic Press, Inc.) |
