| Main Title |
Isolation of a Cytochrome P-450 Structural Gene from 'Saccharomyces cerevisiae'. |
| Author |
Kalb, V. F. ;
Loper, J. C. ;
Dey, C. R. ;
Woods, C. W. ;
Sutter, T. R. ;
|
| CORP Author |
Cincinnati Univ., OH. Coll. of Medicine.;Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab. |
| Publisher |
c1986 |
| Year Published |
1986 |
| Report Number |
EPA-R-810605; EPA/600/J-93/330; |
| Stock Number |
PB93-229482 |
| Additional Subjects |
Cytochrome P450 ;
Structural genes ;
Saccharomyces cerevisiae ;
Drug resistance ;
Ketoconazole ;
Genetic vectors ;
Plasmids ;
Open reading frames ;
Heme ;
Molecular cloning ;
Antibodies ;
Recombinant DNA ;
Genetic translation ;
Genetic transformation ;
Molecular sequence data ;
Reprints ;
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB93-229482 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
11p |
| Abstract |
A Saccharomyces cerevisiae host with an S. cerevisiae genomic library contained in the shuttle vector YEp24 was transformed and the resultant transformants were screened for resistance to ketoconazole (Kc), an inhibitor of the cytochrome P-450 (P-450) enzyme lanosterol 14 alpha-demethylase. Two plasmids were isolated which transformed yeast to both increased resistance to Kc and increased levels of total P-450. Hybrid-selection and immunoprecipitation experiments showed that these plasmids, pVK1 and pVK2, contained the structural gene for an S. cerevisiae P-450. This conclusion was confirmed by the nucleotide sequence of a portion of pVK2, which revealed an open reading frame encoding a characteristic P-450 heme-binding region. |
