The test material was Al 1 sample of the test substance was received from the principal on January 22, 1980. It was a clear colourless liquid, designated: dipropylene glycol. Test animals : Young adult albino rats (Wistar-derived) from the Institute's colony were used, The body weights of males varied from 182 to 283g, those of females from 110 to 164 g. The rats were housed in groups of five in screen- bottomed stainless steel cages in a well-ventilated room, maintained at 23 + or - 1 deg C. Before dosing the rats were fasted overnight. Experimental design : After some preliminary observations the test material was given by gavage to groups of five males and five females in single doses of 8,6, 10,4, 12,4, 14,9 or 17,9 ml per kg body weight. After treatment the rats received stock diet and tap water ad libitan. They were observed for signs of intoxication during a 14-day period, after which autopsies were carried out on the survivors. The LDSO was calculated according to the method of Neil (Biometrics 8 (1952) 249-263). Results: Within a few hours after dosing, the rats showed sedation and signs of ataxia. Later on coma was frequently observed. Deaths occurred between 6 hours and three days after treatment. Then the survivors recovered gradually and looked quite healthy at the end of the observation period.