The dermal sensitization potential of diethylamine (CAS No. 109-89-7) was evaluated in 10 CF1(BR) mice inducted with 3 daily 0.1 mL topical applications of a 1.0% (v/v) test solution to clipped abdomens. A group of 10 mice likewise inducted over 3 days with 0.1 mL dermal applications of 0.5% DNCB served as the positive control. Challenge and rechallenge were administered 7 and 14 days later in dermal applications of a 50% solution to both dorsal and ventral surfaces of the left and right ears, respectively, of inducted test mice. Ear thickness measured at 24 and 48-hour evaluations indicated the degree of sensitization. Challenge and rechallenge elicited no response in either test or irritation control groups at 24 or 48-hour evaluations. Conversely, 80% and 100% positive response in the DCNB-inducted and challenged groups at 24-hour and 48-hour evaluations, respectively, confirmed the validity of the test system.