Record Display for the EPA National Library Catalog


Main Title Embryonic Palatal Responses to Teratogens in Serum-Free Organ Culture.
Author Abbott, B. D. ; Buckalew, A. R. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC. Developmental Toxicology Div. ;ManTech Environmental Technology, Inc., Research Triangle Park, NC.
Publisher c1992
Year Published 1992
Report Number EPA/600/J-92/190;
Stock Number PB92-188960
Additional Subjects Teratogenic compounds ; Organ culture ; Palate ; Embryo ; Serum-free media ; Dose-response relationships ; In vivo analysis ; In vitro analysis ; Tretinoin ; Triamcinolone ; Tetrachlorodibenzodioxins ; Hydrocortisone ; Dexamethasone ; Reprints ;
Library Call Number Additional Info Location Last
NTIS  PB92-188960 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 17p
The study examines development of rat, mouse, and human embryonic palates in submerged, serum-free organ culture. The concentration-response profiles for retinoic acid (RA), triamcinolone (TRI), hydrocortisone (HC), dexamethasone (DEX), and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) were examined and the mechanisms of clefting in vitro were compared to observed in vivo responses. The present study demonstrates that serum-free organ culture supports development of mouse, rat, and human palatal explants. The present study also demonstrates the capacity of this organ culture system to model palatogenesis for several species, and to distinguish between various mechanisms of clefting as presented through selected model compounds. This model should be useful for exploring mechanisms of activity at a cellular and molecular level.