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RECORD NUMBER: 83 OF 131

Main Title Kinetic Studies of the Oxidative Degradation of Detergent and Related Species in Aqueous Solution. I. Microbiological Studies.
Author Ripi, Marilyn J. ; Noo, Kerry F. ; Coo, Thomas M. ;
CORP Author Maryland Univ., Baltimore. Dept. of Microbiology.
Year Published 1970
Report Number OWRR-A-009-MD; 08029,; A-009-MD(1)
Stock Number PB-199 365
Additional Subjects ( Sulfonates ; Biodeterioration) ; ( Detergents ; Biodeterioration) ; ( Bacteria ; Detergents) ; ( Aquatic microbiology ; Detergents) ; Enzymes ; Models ; Surfactants ; Pseudomonas ; Oxidation ; Pyrocatechois ; Microbiology ; Water quality ; Pseudomonas testosteroni
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NTIS  PB-199 365 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 78p
Abstract
A model simulating bacterial degradation of LAS detergents was devised by employing simpler compounds, benzene sulfonate and p-toluene sulfonate, as the sole sources of carbon and sulfur. This substitution permitted observation of alkylarylsulfonate oxidation by a single species of bacteria, identified as Pseudomonas testosteroni. The recorded features of the biodegradation process included effect of substrates and buffers, growth kinetics, cells' respiratory activity, and the influence of inhibitors. Results suggested that catechol or corresponding alkyl derivatives form intermediate products, and that the reaction involves participation of at least one enzyme, catechol-2,3-dioxygenase. Oxidation of catechol via a meta cleavage enzyme has been demonstrated with whole cells, as well as cell-free extracts. Biodegradation of linear alkylate sulfonate detergents was achieved only with mixed bacterial cultures. (Wilde-Wisconsin)