Record Display for the EPA National Library Catalog

RECORD NUMBER: 123 OF 131

Main Title Use of Enzyme-Linked Immunosorbent Assays (ELISA) for the Determination of Triton X Nonionic Detergents.
Author Wie, S. I. ; Hammock., B. D. ;
CORP Author California Univ., Davis.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1982
Report Number EPA-R-806447; EPA/600/J-82/465;
Stock Number PB86-219029
Additional Subjects Immunosorbent assays ; Detergents ; Enzyme linked immunosorbent assays ; Nonionic detergents
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NTIS  PB86-219029 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 11p
Abstract
An enzyme-linked immunosorbent assay (ELISA) for 4-t-octylphenyl ethoxylates such as Triton X-100 was developed. Both the 4-t-octylphenyl and the ethoxylate moiety were required for antibody recognition since members of the Triton N series showed low cross-reactivity, and polyethylene glycol polymers as well as a variety of other neutral and ionic surfactants and pesticides showed no cross-reactivity. The ELISA was sensitive in the low nanogram-per-milliliter range and was highly reproducible. It was shown to be capable of analyzing the active ingredients in vaginal contraceptives and Triton X-100 in the presence of proteins. Immunoassays thus offer advantages in the analysis of such complex mixtures.