Main Title |
Use of Enzyme-Linked Immunosorbent Assays (ELISA) for the Determination of Triton X Nonionic Detergents. |
Author |
Wie, S. I. ;
Hammock., B. D. ;
|
CORP Author |
California Univ., Davis.;Health Effects Research Lab., Research Triangle Park, NC. |
Year Published |
1982 |
Report Number |
EPA-R-806447; EPA/600/J-82/465; |
Stock Number |
PB86-219029 |
Additional Subjects |
Immunosorbent assays ;
Detergents ;
Enzyme linked immunosorbent assays ;
Nonionic detergents
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
NTIS |
PB86-219029 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
Collation |
11p |
Abstract |
An enzyme-linked immunosorbent assay (ELISA) for 4-t-octylphenyl ethoxylates such as Triton X-100 was developed. Both the 4-t-octylphenyl and the ethoxylate moiety were required for antibody recognition since members of the Triton N series showed low cross-reactivity, and polyethylene glycol polymers as well as a variety of other neutral and ionic surfactants and pesticides showed no cross-reactivity. The ELISA was sensitive in the low nanogram-per-milliliter range and was highly reproducible. It was shown to be capable of analyzing the active ingredients in vaginal contraceptives and Triton X-100 in the presence of proteins. Immunoassays thus offer advantages in the analysis of such complex mixtures. |