Record Display for the EPA National Library Catalog
RECORD NUMBER: 21 OF 49| Main Title | Formation of DNA Adducts in 1OT1/2 Mouse Embryo Fibroblasts Incubated with Benzo(a)pyrene or Dihydrodiol Oxide Derivatives. | |||||||||||
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| Author | Brown, Halina Szejnwald ; Jeffrey, Alan M. ; Weinstein, I. Bernard ; | |||||||||||
| CORP Author | Columbia Univ., New York.;Health Effects Research Lab., Research Triangle Park, NC.;National Cancer Inst., Bethesda, MD. | |||||||||||
| Year Published | 1979 | |||||||||||
| Report Number | EPA-R-805482 ;PHS-CA-13696; EPA-600/J-79-148; | |||||||||||
| Stock Number | PB83-145292 | |||||||||||
| Additional Subjects | Deoxyribonucleic acid ; Aromatic polycyclic hydrocarbons ; Binding ; Mice ; Embryos ; Oxygen organic compounds ; Stereochemistry ; Reprints ; Dihydrodiol oxide ; Metabolites ; Cell lines ; Benzopyrenes ; Fibroblasts | |||||||||||
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| Collation | 7p | |||||||||||
| Abstract | Binding of benzo(a)pyrene (BP) metabolites to DNA was studied in the mouse embryo fibroblast 10T one half cell line. The cells metabolized BP to its diastereoisomeric 7,8-dihydrodiol-9,10-oxides, (+)-7B, 8a-dihydroxy-9B, 10B-epoxy-7,8,9,10-tetrahydrobenzo(a)pyrene, which formed covalent adducts with cellular DNA. Detailed analysis by high-pressure liquid chromatography indicated that a deoxyguanosine adduct, N2-(10S-(7R, 8S, 9R-trihydroxy-7,8,9,10-tetrahydrobenzo(a)pyrene)yldeoxyguaninosine, was the predominant DNA adduct formed in cells exposed to BP. This adduct is also the major one found in human tissues exposed to BP. |