| Contents Notes |
pt. I. The organism, community resources, and genome-wide studies. The secret lives of dictyostelium / Richard H. Kessin -- the genome of dictyostelium discoideum / Adam Kuspa and William F. Loomis -- The cDNA sequencing project / Hideko Urushihara, Takahiro Morio, and Yoshimasa Tanaka -- dictyBase and the dicty stock center / Petra Fey ... [et al.] -- Analysis of gene expression using cDNA microarrays / Marcel Kaul and Ludwig Eichinger -- Proteomic analysis of dictyostelium discoideum / Udo Roth, Stefan Mèuller, and Franz-Georg Hanisch -- pt. II. Basic methods and molecular genetic techniques. Cultivation, spore production, and mating / Hideko Urushihara -- Parasexual genetics using axenic cells / Jason King and Robert Insall -- Slug phototaxis, thermotaxis, and spontaneous turning behavior / Paul R. Fisher and Sarah J. Annesley -- Purification techniques of subcellular compartments for analytical and preparative purposes / Laurence Aubry and Gâerard Klein -- Generation of multiple knockout mutants using the cre-loxP system / Alan r. Kimmel and Jan Faix. Restriction enzyme-mediated integration (REM1) mutagenesis / Adam Kuspa -- RNA interference and antisense-mediated gene silencing in dictyostelium / Markus Kuhlmann, Blagovesta Popova, and Wolfgang Nellen -- pt. III. Imaging and localization methods. Application of fluorescent protein tags as reporters in live-cell imaging studies / Annette Mèuller-Taubenberger -- Investigating gene expression: in situ hybridization and reporter genes / Ricardo Escalante and Leandro Sastre -- Application of 2D and 3D DIAS to motion analysis of live cells in transmission and confocal microscopy imaging / Deborah Wessels, Spencer Kuhl, and David R. Soll -- Using quantitative fluorescence microscopy and FRET imaging to measure spatiotemporal signaling events in single living cells / Xuehua Xu, Joseph A. Brzostowski, and Tian Jin -- Visualizing signaling and cell movement during the multicellular stages of dictyostelium development / Dirk Dormann and Cornelis J. Weijer. Under-agarose chemotaxis of dictyostelium discoideum / David Woznica and David A. Knecht -- Optimized fixation and immunofluorescence staining methods for dictyostelium cells / Monica Hagedorn, Eva M. Neuhaus, and Thierry Soldati -- Cryofixation methods for ultrastructural studies of dictyostelium discoideum / Mark J. Grimson and Richard L. Blanton -- pt. IV. Dictyostelium as model organism. Analysis of signal transduction: formation of cAMP, cGMP, and Ins(1,4,5)P, in vivo and in vitro / Peter J.M. Van Haastert -- Assaying chemotaxis of dictyostelium cells / Michelle C. Mendoza and Richard A. Firtel -- Characterization of cross-linked actin filament gels and bundles using birefringence and polarized light scattering / Ruth Furukawa and Marcus Fechheimer -- Quantitative and microscopic methods for studying the endocytic pathway / Francisco Rivero and Markus Maniak. Preparation of intact, highly purified phagosomes from dictyostelium / Daniel Gotthardt ... [et al.] -- Assaying cell-cell adhesion / Salvatore Bozzaro -- Periodic activation of ERK2 and partial involvement of G protein in ERK2 activation by cAMP in dictyostelium cells / Mineko Maeda -- An improved method for dictyostelium centrosome isolation / Irene Schulz ... [et al.] -- Epigenetics in dictyostelium / Markus Kaller, wolfgang Nellen, and Jonathan R. Chubb -- Dictyostelium discoideum as a model to study host-pathogen interactions / Can èUnal and Michael Steinert -- Pharmacogenetics: defining the genetic basis of drug action and inositol trisphosphate analysis / Kathryn E. Adley, Melanie Keim, and Robin S.B. Williams -- How to assess and study cell death in dictyostelium discoideum / Artemis Kosta ... [et al.]. |
