Cell lines -- Initiation and maintenance of cultures -- Characterizing cells in culture -- Production and maintenance of hybridomas -- Cell separation -- Culture media -- Supplements to basal media -- Balanced salt solutions and buffers -- Cryopreservation -- Microcarriers and microspheres -- Growth factors and other nonnutrient factors -- Analysis of culture media -- Products of commercial value from normal and genetically engineered cells -- Culture vessels and bioreactors -- Safety aspects of handling cells.