Record Display for the EPA National Library Catalog


Main Title Primary Structure of the Cytochrome P450 Lanosterol 14 alpha-Demethylase Gene from 'Candida tropicalis'.
Author Chen, C. ; Kalb, V. F. ; Turi, T. G. ; Loper, J. C. ;
CORP Author Cincinnati Univ., OH. Coll. of Medicine.;Environmental Protection Agency, Cincinnati, OH. Risk Reduction Engineering Lab.
Publisher c1988
Year Published 1988
Report Number EPA-R-810605 ;EPA-R-813366; EPA/600/J-93/326;
Stock Number PB93-229441
Additional Subjects Amino acid sequence ; Fungal genes ; Saccharomyces cerevisiae ; Amino acid sequence homology ; Deoxyribonucleic acids ; Open reading frames ; Reprints ; Candida tropicalis ; Cytochrome P450 lanosterol 14 alpha-demethylase
Library Call Number Additional Info Location Last
NTIS  PB93-229441 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 13p
The nucleotide sequence of the gene and flanking DNA for the cytochrome P450 lanosterol 14 alpha-demethylase (14DM) from the yeast Candida tropicalis ATCC750 is reported. An open reading frame (ORF) of 528 codons encoding a 60.9-kD protein is identified. This ORF includes a characteristic heme-binding domain, HR2, common to all P450 proteins. This protein and the 14DM from Saccharomyces cerevisiae share 66.5% identical and 23.1% conservatively replaced amino acids in a 516-amino-acid alignment, and thus are orthologous forms of the P450LIA1 gene. Conversely, C. tropicalis 14DM shares relatively little sequence similarity with P450alk, the predominant P450 protein present when this organism is grown on n-alkanes. Sequence information of these three yeast P450s will be useful for structure-function analyses in the future.