| Main Title |
Comparison of In vivo and In vitro Methods for Assessing the Effects of Carbon Tetrachloride on the Hepatic Drug-Metabolizing Enzyme System. |
| Author |
Chadwick, R. W. ;
Copeland, M. F. ;
Carlson, G. P. ;
Trela, B. A. ;
Most, B. M. ;
|
| CORP Author |
Health Effects Research Lab., Research Triangle Park, NC. ;Northrop Services, Inc., Research Triangle Park, NC. ;Purdue Univ., Lafayette, IN. Dept. of Pharmacology and Toxicology. |
| Publisher |
c1988 |
| Year Published |
1988 |
| Report Number |
EPA/600/J-88/545; |
| Stock Number |
PB91-109264 |
| Additional Subjects |
Toxicology ;
Carbon tetrachloride ;
Liver ;
Chlorobenzenes ;
Enzymes ;
In vitro analysis ;
In vivo analysis ;
Metabolism ;
Insecticides ;
Solvents ;
Reprints ;
UDP glucuronosyltransferase ;
Sulfotransferases ;
Glutathione transferases ;
Cytochrome P-450
|
| Holdings |
| Library |
Call Number |
Additional Info |
Location |
Last
Modified |
Checkout
Status |
| NTIS |
PB91-109264 |
Some EPA libraries have a fiche copy filed under the call number shown. |
|
07/26/2022 |
|
| Collation |
10p |
| Abstract |
The effect of a single i.p. injection of 0, 20, 200, and 1000 microliters/kg carbon tetrachloride on the activity of the hepatic drug-metabolizing enzyme system was measured in the rat by a model substrate assay, employing lindane (gamma-hexachlorocyclohexane), and by a battery of in vitro enzyme assays. The data in the study indicated that carbon tetrachloride had a biphasic influence on the phase I reactions with the lowest dose inducing a significant increase in enzyme activity while the highest dose produced significant inhibition. Significant CCl4-induced reductions in glucuronyltransferase and sulfotransferase activities were also observed while the effect on glutathione-S-transferase was ambiguous. The in vivo and in vitro assays showed good agreement. |
