Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Cytochemical Methods for Assessing 'Giardia' Cyst Viability.
Author Lindmark, D. G. ;
CORP Author Cleveland State Univ., OH. Dept. of Biology.;Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1987
Report Number EPA-R-811949; EPA/600/1-87/011;
Stock Number PB88-124748
Additional Subjects Giardiasis ; Parasitic diseases ; Gastrointestinal diseases ; Epidemiology ; Toxicology ; Air pollution ; Water pollution ; Hazardous wastes ; Cytochemistry ; Toxic substances ; Environmental monitoring
Library Call Number Additional Info Location Last
NTIS  PB88-124748 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/21/1988
Collation 69p
Giardia lamblia is the most common human intestinal protozoan parasite reported in the United States and England. The objective of the study was to develop a practical, reliable, rapid, microscopically-read method, comparable to excystation for determining the viability of Giardia cysts. At first an attempt was made to use methods dependent on cyst metabolism such as the activity of pyruvate: ferredoxin oxidoreductase (PFOR) and cell respiration. Though they gave promising results with trophozoites, they proved unreliable with cysts. Hence, used was a fluorescent dye exclusion technique previously proved to be successful for measuring viability in mammalian cells.