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OLS Field Name OLS Field Data
Main Title Interactive Effects of Aldrin, Cyclohexylamine, 2,4-Diaminotoluene and Two Phorbol Esters on Metabolic Cooperation between V79 Cells.
Author Mills, L. J. ; Robson, D. L. ; Malcolm, A. R. ;
CORP Author Science Applications International Corp., Narragansett, RI.;Environmental Research Lab., Narragansett, RI.
Publisher c1991
Year Published 1991
Report Number EPA-68-C1-0005 ;EPA-68-03-3529; EPA/600/J-91/233 ; ERLN-1217
Stock Number PB92-108026
Additional Subjects Carcinogens ; Phorbol esters ; Aldrin ; Cyclohexylamines ; Metabolism ; Intercellular junctions ; Chinese hamsters ; Cell line ; Dose-response relationships ; Reprints ; Diaminotoluenes
Holdings
Library Call Number Additional Info Location Last
Modified
Checkout
Status
NTIS  PB92-108026 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 02/24/1992
Collation 9p
Abstract
The in vitro V79/metabolic cooperation assay measures the extent of gap-junctional transfer of metabolites from wild-type to mutant V79 cells. The assay is currently being explored as a short-term test to screen for tumor promoting chemicals, many of which inhibit metabolic cooperation. In the study, the assay was used to determine whether chemical interactions affect detection of tumor promoters in mixtures and to investigate types of interactions that may occur between chemicals. Several two-chemical mixtures were examined. The effects of phorbol-12-myristate-13-acetate (PMA) and phorbol-12,13-dibutyrate, two inhibitors of metabolic cooperation that operate through the same receptor-mediated pathway, were additive at concentrations below the maximally effective concentrations of either. A summation effect was observed in mixtures of two other inhibitors of metabolic cooperation, the pesticide aldrin and the principal metabolite of sodium cyclamate, cyclohexylamine.