||Interactive Effects of Aldrin, Cyclohexylamine, 2,4-Diaminotoluene and Two Phorbol Esters on Metabolic Cooperation between V79 Cells.
Mills, L. J. ;
Robson, D. L. ;
Malcolm, A. R. ;
||Science Applications International Corp., Narragansett, RI.;Environmental Research Lab., Narragansett, RI.
||EPA-68-C1-0005 ;EPA-68-03-3529; EPA/600/J-91/233 ; ERLN-1217
Phorbol esters ;
Intercellular junctions ;
Chinese hamsters ;
Cell line ;
Dose-response relationships ;
||Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy.
The in vitro V79/metabolic cooperation assay measures the extent of gap-junctional transfer of metabolites from wild-type to mutant V79 cells. The assay is currently being explored as a short-term test to screen for tumor promoting chemicals, many of which inhibit metabolic cooperation. In the study, the assay was used to determine whether chemical interactions affect detection of tumor promoters in mixtures and to investigate types of interactions that may occur between chemicals. Several two-chemical mixtures were examined. The effects of phorbol-12-myristate-13-acetate (PMA) and phorbol-12,13-dibutyrate, two inhibitors of metabolic cooperation that operate through the same receptor-mediated pathway, were additive at concentrations below the maximally effective concentrations of either. A summation effect was observed in mixtures of two other inhibitors of metabolic cooperation, the pesticide aldrin and the principal metabolite of sodium cyclamate, cyclohexylamine.