Record Display for the EPA National Library Catalog


OLS Field Name OLS Field Data
Main Title Effect of Removal of the Cytolytic Factor of 'Bacillus thuringiensis' Subsp. 'Israelensis' on Mosquito Toxicity.
Author Held, G. A. ; Huang, Y. S. ; Kawanishi, C. Y. ;
CORP Author Health Effects Research Lab., Research Triangle Park, NC.
Year Published 1986
Report Number EPA/600/J-86/350;
Stock Number PB87-184693
Additional Subjects Culicidae ; Peptides ; Toxicity ; Larvae ; Pesticides ; Proteins ; Hemolysis ; Bioassay ; Reprints ; Bacillus thuringiensis israelensis ; Monoclonal antibodies
Library Call Number Additional Info Location Last
NTIS  PB87-184693 Most EPA libraries have a fiche copy filed under the call number shown. Check with individual libraries about paper copy. 06/21/1988
Collation 8p
Solubilized crystal protein of Bacillus thuringiensis subsp. israelensis was fractionated by affinity chromotography using a monoclonal antibody directed against the crystal's 28 kDa peptide. The 28 kDa peptide ws found to be relatively nontoxic to mosquito larvae although it does contain the hemolytic activity of the crystals. The crystal protein fraction depleted of the 29 kDa peptide was found to be nonhemolytic and to retain nearly full toxicity to mosquito larvae. These results suggest that the 28 kDa peptide is not required for the toxicity of solubilized crystal protein.