Record Display for the EPA National Library Catalog


Main Title Use of the mixed flask culture (MFC) microcosm protocol to estimate the survival and effects of microorganisms added to freshwater ecosystems /
Author Shannon, Lyle. ; Anderson, R. L.
Other Authors
Author Title of a Work
Anderson, Richard L.
CORP Author Environmental Research Lab.-Duluth, MN. ;Minnesota Univ.-Duluth. Dept. of Biology.
Publisher U.S. Environmental Protection Agency, Office of Research and Development, Environmental Research Laboratory,
Year Published 1989
Report Number EPA/600/D-89/058
Stock Number PB89-221295
Additional Subjects Toxic substances ; Fresh water biology ; Aquatic microbiology ; Cultures(Biology) ; Dissolved gases ; Oxygen ; pH ; Food chains ; Culicidae ; Genetic engineering ; Biological pest control ; Aquatic ecosystems ; Environmental tests ; Water microbiology ; Environmental persistence
Library Call Number Additional Info Location Last
NTIS  PB89-221295 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 37 pages ; 28 cm
Live microorganisms present a special problem when assessing their effects in natural systems. Some determination of the probability of the test organisms' survival in the wild must be made and laboratory microcosms offer an appropriate solution. The report describes a microcosm test procedure based on the concept that many ecosystem processes are independent of species composition. As long as suitable groups are represented in the microcosms, these processes will occur and a viable, self-sustaining community will develop. Using the procedures outlined in this protocol 3 tests of chemicals and 4 tests with micoorganisms have been completed . The report presents the procedures and provides examples of data from experiments.
"EPA/600/D-89/058." "To be submitted to: American Society of Microbiologists." Microfiche.