Record Display for the EPA National Library Catalog


Main Title Benzoate-Dependent Induction from the OP2 Operator-Promoter Region of the TOL pWWO in the Absence of Known Plasmid Regulatory Genes (Journal Version).
Author Cuskey, S. M. ; Sprenkle, A. B. ;
CORP Author Environmental Research Lab., Gulf Breeze, FL. ;Technical Resources, Inc., Gulf Breeze, FL.
Publisher c1988
Year Published 1988
Report Number EPA/600/J-88/170;
Stock Number PB89-119432
Additional Subjects Enzyme induction ; Oxygenases ; Operon ; Microbial genetics ; Gene expression regulation ; Molecular cloning ; Genetic transformation ; Benzoates ; Plasmids ; Reprints ; Pseudomonas putida
Library Call Number Additional Info Location Last
NTIS  PB89-119432 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 7p
The OP2 (Pm) operator-promoter region of the TOL plasmid, pWWO, is located immediately upstream from the 'lower' pathway operon responsible for the catabolism of benzoate and related aromatic acids. Induction of the operon in the presence of aromatic acid inducers, requires the xylS gene product. The authors cloned a 401 base pair region containing the OP2 region upstream from the promoter less xylE (catechol 2,3-dioxygenase) gene and measured induction in the presence of a variety of aromatic inducers in a xylS free background. Approximately 30-fold induction was seen when transformed cells of Pseudomonas putida strain PRS2000 were grown on lactate in the presence of benzoate. Slight (4-fold) induction occurred when m-toluic acid was included in the growth medium.