Record Display for the EPA National Library Catalog


Main Title Cloning, Expression, and Regulation of the 'Pseudomonas cepacia' Protocatechuate 3,4-Dioxygenase Genes.
Author Zylstra, G. J. ; Olsen, R. H. ; Ballou, D. P. ;
CORP Author Michigan Univ., Ann Arbor. Medical School.;Environmental Research Lab., Gulf Breeze, FL.
Publisher cNov 89
Year Published 1989
Report Number EPA/600/J-89/435;
Stock Number PB90-264771
Additional Subjects Pseudomonas ; Mutations ; Reprints ; Molecular cloning ; Gene expression ; Protocatechuate 3-4-dioxygenase ; Bacterial genes ; Plasmids ; Restriction mapping
Library Call Number Additional Info Location Last
NTIS  PB90-264771 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 10p
Genes for the alpha and beta subunits of the enzyme protocatechuate 3,4-dioxygenase were cloned from the Pseudomonas cepacia DB01 chromosome on a 9.5 kilobase pair PstI fragment into the broad-host-range cloning vector pR02317. The resultant clone was able to complement protocatechuate 3,4-dioxygenase mutations in P. cepacia, P. aeruginosa, and P. putida. Expression studies showed that the genes were constitutively expressed and subject to catabolite repression in the heterologous host. Since the cloned genes exhibited normal induction patterns when present in P. cepacia DB01 it was concluded that induction was subject to negative control. (Copyright (c) 1989 American Society for Microbiology).