Record Display for the EPA National Library Catalog

RECORD NUMBER: 11 OF 60

Main Title Catabolism of Aromatic Biogenic Amines by 'Pseudomonas aeruginosa' PA01 via meta Cleavage of Homoprotocatechuic Acid (Journal Version).
Author Cuskey, S. M. ; Olsen, R. H. ;
CORP Author Environmental Research Lab., Gulf Breeze, FL. ;Michigan Univ., Ann Arbor. Medical School.
Publisher c1988
Year Published 1988
Report Number EPA/600/J-88/109;
Stock Number PB89-120067
Additional Subjects Biogenic amines ; Pseudomonas aruginosa ; Metabolism ; Bacteria ; Tyramine ; Oxygenases ; NADH ; NADPH oxidoreductases ; Analklyamines ; Mutations ; Enzyme induction ; Molecular cloning ; Chromosome mapping ; Reprints ; Octopamine ; Benzene methanol/(aminomethyl)-hydroxy
Holdings
Library Call Number Additional Info Location Last
Modified		 Checkout
Status
NTIS  PB89-120067 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 8p
Abstract
Pseudomonas aruginosa PA01 catabolized the aromatic amines tyramine and octopamine through 4-hydroxyphenylacetic acid and 3,4-dihydroxyphenylacetic acid (HPA). Meta ring cleavage was mediated by 3-4-dihydroxyphenylacetate 2,3-dioxygenase (HPADO), producing 2-hydroxy-5-carboxymethylmuconic semialdehyde (MSA). An NAD-dependent dehydrogenase caused the disappearance of the yellow MSA product, probably forming 2-hydroxy-5-carboxymethylmuconic acid. Induction studies with extracts from mutant cells indicated that the inducer of HPADO was HPA and/or MSA. Strains PA01.221 (tynCl) and PA01.303 (tynDI) have chromosomal mutations causing a deficiency in the activity necessary for conversion of 4-hydroxyphenylacetic acid to HPA. (Copyright (c) 1988 American Society for Microbiology.)