Record Display for the EPA National Library Catalog
RECORD NUMBER: 1 OF 17Main Title | Catabolism of Aromatic Biogenic Amines by 'Pseudomonas aeruginosa' PA01 via meta Cleavage of Homoprotocatechuic Acid (Journal Version). | |||||||||||
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Author | Cuskey, S. M. ; Olsen, R. H. ; | |||||||||||
CORP Author | Environmental Research Lab., Gulf Breeze, FL. ;Michigan Univ., Ann Arbor. Medical School. | |||||||||||
Publisher | c1988 | |||||||||||
Year Published | 1988 | |||||||||||
Report Number | EPA/600/J-88/109; | |||||||||||
Stock Number | PB89-120067 | |||||||||||
Additional Subjects | Biogenic amines ; Pseudomonas aruginosa ; Metabolism ; Bacteria ; Tyramine ; Oxygenases ; NADH ; NADPH oxidoreductases ; Analklyamines ; Mutations ; Enzyme induction ; Molecular cloning ; Chromosome mapping ; Reprints ; Octopamine ; Benzene methanol/(aminomethyl)-hydroxy | |||||||||||
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Collation | 8p | |||||||||||
Abstract | Pseudomonas aruginosa PA01 catabolized the aromatic amines tyramine and octopamine through 4-hydroxyphenylacetic acid and 3,4-dihydroxyphenylacetic acid (HPA). Meta ring cleavage was mediated by 3-4-dihydroxyphenylacetate 2,3-dioxygenase (HPADO), producing 2-hydroxy-5-carboxymethylmuconic semialdehyde (MSA). An NAD-dependent dehydrogenase caused the disappearance of the yellow MSA product, probably forming 2-hydroxy-5-carboxymethylmuconic acid. Induction studies with extracts from mutant cells indicated that the inducer of HPADO was HPA and/or MSA. Strains PA01.221 (tynCl) and PA01.303 (tynDI) have chromosomal mutations causing a deficiency in the activity necessary for conversion of 4-hydroxyphenylacetic acid to HPA. (Copyright (c) 1988 American Society for Microbiology.) |