Record Display for the EPA National Library CatalogRECORD NUMBER: 8 OF 16
|Main Title||Identification of aneuploidy-inducing agents using the wheat seedling assay /|
|Author||Sandhu, S. S. ; Dhesi, J. S. ; Gill, B. S.|
|CORP Author||Environmental Health Research and Testing, Inc., Research Triangle Park, NC. ;North Carolina Central Univ., Durham.;Health Effects Research Lab., Research Triangle Park, NC. Genetic Toxicology Div.|
|Publisher||U.S. Environmental Protection Agency, Office of Research and Development, Health Effects Research Laboratory,|
|Report Number||EPA/600/D-89/091; EPA-68-02-4456|
|Additional Subjects||Toxicity ; Mutagens ; Wheat plants ; Cyclophosphamide ; Chromium inorganic compounds ; Aneuploidy ; Mutagenicity tests ; Chromosome aberrations ; Alleles ; DNA repair|
|Collation||17 pages ; 28 cm|
A short-term assay using Chinese spring wheat for detecting induced aneuploidy and/or clastogenicity is described. The seed of virescent wheat (v1v1) are treated with a test agent. The appearance of white sectors (v1v1v1) or green sectors (v10) on the virescent leaves indicates the gain or loss of a chromosome or a segment of a chromosome bearing v1 allele. Three test chemicals, chromium trioxide (100-5,000 microg/ml), chromium dichromate (500-5,000 microg/ml), and cyclophosphamide (100-1,000 microg/ml) produced a dose-related positive response for chromosome aberrations in the assay.
EPA/600/D-89/091. PB89-223978. Microfiche.