Record Display for the EPA National Library Catalog
RECORD NUMBER: 35 OF 52| Main Title | Purification of 'Giardia muris' Cysts by a Velocity Sedimentation. | |||||||||||
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| Author | Sauch, J. F. ; | |||||||||||
| CORP Author | Health Effects Research Lab., Cincinnati, OH. | |||||||||||
| Year Published | 1984 | |||||||||||
| Report Number | EPA/600/J-84/160; | |||||||||||
| Stock Number | PB85-146389 | |||||||||||
| Additional Subjects | Cells(Biology) ; Separation ; Microbiology ; Bacteria ; Feces ; Contamination ; Sedimentation ; Centrifuging ; Purification ; Reprints ; Giardia muris | |||||||||||
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| Collation | 5p | |||||||||||
| Abstract | Giardia muris cysts were separated from fecal contaminants in primary isolates using unit gravity velocity sedimentation. Crude isolates were overlayed onto a Percoll density gradient, 1.01 g/cu cm to 1.03 g/cu cm. Giardia muris cysts were well separated from faster-sedimenting fecal debris and slower-sedimenting Spironucleus muris and bacteria in 1.5 hours. |