Main Title |
Molecular cloning : a laboratory manual / |
Author |
Maniatis, Tom,
|
Other Authors |
|
Publisher |
Cold Spring Harbor Laboratory, |
Year Published |
1982 |
OCLC Number |
07976996 |
ISBN |
0879691360; 9780879691363 |
Subjects |
Molecular cloning ;
Eukaryotic cells ;
Clonage molculaire--Méthodes ;
Cellule eukaryote ;
Clones cellulaires ;
Recombinaison génétique ;
Génie génétique ;
Eukaryontische Zelle ;
Genklonierung ;
Methode ;
Pflanzen ;
Ungeschlechtliche Fortpflanzung ;
Genetica ;
Clonage molculaire--Manuels de laboratoire ;
Cellules eucaryotes ;
Cloning, Molecular
|
Additional Subjects |
Eukaryotic cells ;
Molecular cloning
|
Holdings |
Library |
Call Number |
Additional Info |
Location |
Last Modified |
Checkout Status |
EJEM |
QH442.2.M26 1982 |
|
OCSPP Chemical Library/Washington,DC |
06/29/1991 |
|
Collation |
x, 545 pages : illustrations ; 28 cm |
Notes |
Includes bibliographical references (pages 507-520) and index. |
Contents Notes |
1. Vector-host systems -- 2. Propagation and maintenance of bacterial strains and viruses -- 3. Isolation of bacteriophage [lambda] and plasmid DNA -- 4. Enzymes used in molecular cloning -- 5. Gel electrophoresis -- 6. Extraction, purification, and analysis of mRNA from eukaryotic cells -- 7. Synthesis and cloning of cDNA -- 8. Introduction of plasmid and bacteriophage [lambda] DNA into Escherichia coli -- 9. Construction of genomic libraries -- 10. The identification of recombinant clones -- 11. Analysis of recombinant DNA clones -- 12. Vectors that express cloned DNA in Escherichia coli. |