Record Display for the EPA National Library Catalog


Main Title Isolation and Culture of Leydig Cells from Adult Rats.
Author Klinefelter, G. R. ; Kelce, W. R. ; Hardy., M. P. ;
CORP Author Johns Hopkins Univ., Baltimore, MD. School of Hygiene and Public Health. ;Population Council, New York. Center for Biomedical Research.;Health Effects Research Lab., Research Triangle Park, NC. Reproductive Toxicology Branch.
Publisher 1992
Year Published 1992
Report Number EPA-68-02-4450, EPA-R-816056; EPA/600/A-92/071;
Stock Number PB92-166321
Additional Subjects Leydig cells ; Cultured cells ; Testosterone ; Rats ; Sperm motility ; Cell survival ; Culture media ; Toxicology ; Xenobiotics ; In vitro analysis ;
Library Call Number Additional Info Location Last
NTIS  PB92-166321 Some EPA libraries have a fiche copy filed under the call number shown. 07/26/2022
Collation 28p
Testosterone is essential for quantitatively normal sperm production in the testis, normal sperm maturation in the epididymis, maintenance of the accessory sex organs, and effective sexual behavior. A variety of xenobiotics can result in a significant decrease in spermatogensis, sperm motility and fertility, libido, or simply the circulating level of testosterone. Thus, the ability to assess the steroidogenic capacity of the Leydig cell is pivotal to a complete characterization of toxicant-induced effects on reproductive function in the male. Previously, it was impossible to conduct definitive studies to identify direct toxicant-induced effects on Leydig cell function and viability since a method to provide viable, highly purified Leydig cell preparation was unavailable. Herein the authors describe such an isolation procedure as well as criteria for maintaining Leydig cells in primary culture. A primary culture of Leydig cells which maintains function over time, provides a model for those interested in addressing the more mechanistic issues in Leydig cell toxicology and permits the determination of the reversibility of toxicant-induced effects in vitro.